Kompatibilitas Bacillus Subtilis, Pseudomonas Fluorescens, dan Trichoderma Harzianum untuk Mengendalikan Ralstonia Solanacearum pada Tanaman Kentang

摘要

. Hanudin, Marwoto, B, Hersanti, and Muharam, A 2012. Compatibilities of Bacillus subtilis, Pseudomonas fluorescens, and Trichoderma harzianum to Control Ralstonia solanacearum on Potato. Among the vegetable crops cultivated by farmers, potato is the most important one. In cultivating the crop, farmers has faced many problems, the most important one is wilt disease caused by Ralstonia solanacearum E. F. Smith. Based on the available data known that the disease reduces 40–100% of the total production. One of the alternative methods control which was more environmentally friendly applied by the use of antagonistic microbes isolated from the soil. Bacillus subtilis, P. fluorescens, and T. harzianum were abundance in the soil and easily be isolated from rhizosfer. The bacteria are reported to be effective to control soilborn pathogens in the field. Based on the preliminary research proven that the bacteria could reduce 70% of the total soilborn pathogens in the soil and increase crop production up to 40%. The objective of this study was to determine information of compatibilities between antagonistic microbe and bacterial wilt which had control on potato. The research were conducted in Laboratory and Greenhouse of the Padjadjaran University, Indonesian Vegetable Research Institute, and Indonesian Ornamental Crops Research Institute, on March to December 2009. The results showed that isolates of P. fluorescens (Pf2), B. subtilis (Bs12), and T. harzianum (Th1) were compatible on medium which containing protein (King\u27s B), but were not copmatible on medium containing carbohydrate (PDA). The indicator is compatibility index ≤1. Single application of B. subtilis was effective to control R. solanacearum on potato, suppression wilt biggest (35,27%), if compared with other treatments except combination between Bs 12, Pf 2, and Th 1 treatments was 35,27 % too.